Monday, 4 July 2016

HLA-Typed Haematopoietic Cells

Did you know that AllCells provide HLA typed PBMCs?  

AllCells specializes in the provision of haematopoietic and immunological cells collected from healthy donors within an IRB Approved Clinical Division. This allows the scheduling of all donors and the capability to recall donors to meet your specific HLA typing needs. We currently provide both low and high resolution HLA typing options and have a large selection of PBMC's in stock with known HLA types.  Customers receive HLA Donor Reports specific to the HLA typed lots purchased.  HLA fees vary depending on frozen or fresh product requirements and are applied on a per donor basis.  Let AllCells be your expert resource for HLA typed donors.

An example of the products that have currently been HLA typed, and the donor information that can be provided can be found here. Please contact me to discuss your requirements in further detail or for pricing information.

Wednesday, 29 June 2016

DNA Methylation Research Just Got Easier

DNA Methylation Research Just Got Easier
Caltag Medsystems
Accurately Measure Global 5-mC or 5-hmC in DNA in Just 2 Hours!
Now you can save yourself the extra effort and quantify global 5-mC or 5-hmC levels in DNA in just one easy step with Epigentek’s MethylFlash™ Global DNA Methylation and Hydroxymethylation ELISA Easy Kits. These two new kits allow you to quantify global DNAmethylation or hydroxymethylation status by specifically measuring levels of 5-mC or 5-hmC in a simplified, "one-step" ELISA-like format. 
Advantages & Features of MethylFlash Easy Kits:
  • Fast - Reduced steps allow you to finish in only 2 hours
  • Robust - Improved kits have a greater “signal window” with reduced variation between replicates
  • Convenient - Inherently low background noise without the need for DNA denaturation and plate blocking steps
  • Sensitive - Detection limit can be as low as 0.05% methylated or 0.01% hydroxymethylated DNA from 100 ng of input
  • DNA Specific - No cross-reactivity to unmethylated or other modified cytosines
  • Universal - Included positive and negative controls allow for detection of DNA methylation or hydroxymethylation in any species from either single-stranded or double-stranded input
  • DNA Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assays to be more accurate and highly comparable with HPLC-MS analysis
  • Flexible - Strip-well microplate format for manual or high throughput analysis
Plus, save 25% for a limited time! 
How are these new kits different from the previous MethylFlash kits?
The MethylFlash Easy kits, while still retaining the many advantageous features of the previous kits, utilises a “one-step” ELISA reaction to eliminate the need for multiple sample and antibody binding steps. The protocols of these new kits are simplified, reducing the procedure times by 50% versus the previous MethylFlash kits. Some adjustments were made to also reduce the CV% between wells. The greater “signal window” of each kit, along with adjusted calculation formulas, makes these new kits very accurate. In a nutshell, MethylFlash Easy kits are faster, easier, more robust, and more accurate.
Epigentek products are distributed in the UK by Caltag Medsystems Ltd.

Monday, 27 June 2016

Live Webinar: A New Dimension for Adipocyte Culture

Live Webinar: A New Dimension for Adipocyte Culture
Caltag Medsystems
Cell Culture Mimicking the Fat Cell Microenvironment
Presented by Celenys
Monday 4th July, 15.00 BST
BIOMIMESYS®Adipocyte represents a new generation of mimetic hydrogel for 3D adipocyte culture. Available in a ready-to-use format it enables the culture of adipocytes and adipocyte-like cells under physiological conditions that are representative of the microenvironment found in adipose tissue. The highly porous nature of the scaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D adipocyte cultures.
- 2D versus 3D cell culture
- Comparison of 3D cell culture techniques
- Hyaluronic acid scaffold for 3D cell culture
- Functional scaffolds: adipocyte
- Q&A

Wednesday, 22 June 2016

Newly Added: Human Liver Mononuclear Cells

Human Liver Mononuclear Cells
Cells | Stem Cells | Media | Reagents | Growth Factors | Assay Kits | Gene Analysis
Human Liver Mononuclear Cells
Human Liver Mononuclear Cells (HLMC) are a mixed population of cells that include T-lymphocytes, B-lymphocytes, NKcells, and monocytes. HLMC are isolated from the human liver, depleted of erythrocytes and hepatic macrophages, cryopreserved immediately after isolation, and delivered frozen.
HLMC are an excellent model to study the innate and adaptive immune system.
It is recommended to use HematoGro Medium (Cat. #5501) for short-term maintenance.

Tuesday, 24 May 2016

Angiogenesis: Analyse the Complete Pathway

Angiogenesis: Analyse the Complete Pathway

V2a Kit™ - Vasculogenesis to Angiogenesis
An in vitro human primary cell co-culture model of angiogenesis that includes all the phases of the angiogenic process.
Tubules formed contain a lumen and resemble the morphology and range of capillary lengths found in microvascular beds in vivo.

Build a Bespoke Angiogenesis Assay
Cellworks Angiogenesis tested Human Umbilical Vein Endothelial Cells (HUVEC) and Human Dermal Fibroblasts (HDF) in combination produce tubules.
Cells, medium packages and staining kits are available separately, allowing total flexibility in angiogenesis assay design.
This option provides complete control over the number and cell types present, and the opportunity to manipulate the cells prior to culturing, for example by transfection.

Angiogenesis Image Analysis Software
The AngioSys 2.0 image analysis software provides a semi-automated analysis of angiogenesis by measuring the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each image.
Angiosys 2.0 is custom built software developed to quantitate tubules formed during in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts.

Angiogenesis Complete Assay Service
Analyse the Angiogenic Pathway: Cellworks Angiogenesis Service.
Compounds, media and tissue explants can be tested at any point in the angiogenic pathway using the Cellworks Angiogenesis Service. The service includes the following stages and can be tailored to your needs:
Performing Angiogenesis AssaysCapture and Analysis of Images
Provision of Raw Data
Bespoke Options

Friday, 20 May 2016

Non-Foetal Human Cells Available

Caltag Medsystems
Non-Foetal Human Cells Available
Caltag Medsystems

Caltag Medsystems
 has access to a large number of human cell types of non-foetal origin, including fibroblasts, cardiomyocytes, stellates and haematological cells. 
Contact us now to find out if we have the cells that you require.
 Merge Organ Networks in the SciFlow System

SciFlow™ dynamic culture system incorporates physiological fluid flow to any 2D or 3D tissue culture assay system. Controlled fluid flow improves the physiological relevance of existing cell-based assays and allows for new cell-based assays to be developed within a connected environmental system.
  • Connect multiple different tissues in series
  • Perform more life-like drug exposures in a user-configurable system that mimic complete target organ environments
  • Maintains a small footprint with virtually no set up cost to user
  • Readily adaptable to existing safety and toxicity assays
  • Compatible with most existing laboratory instrumentation.
Caltag Medsystems - your cost efficient supplier of the SciFlow system. 
+44 (0)1280 827460

Monday, 9 May 2016

Cloud - Clone : Your Immunoassay Provider

Your Immunoassay Provider
Cloud Clone header
Cloud-Clone are a specialist manufacturer of ELISA KitsCLIA Kits, Polyclonal and MonoclonalAntibodies, and Recombinant Proteins. With over 33,000 products, across a wide range of research areas, you are sure to find a product of interest. 
To celebrate becoming the exclusive Cloud-Clone distributor in the UK and Ireland, we are pleased to offer:
- Price reductions across the whole Cloud-Clone range
- Additional pack sizes
- Loading controls now included with every antibody purchased

Download the following spreadsheets to find the full range of Cloud-Clone products:

If you cannot find what you're looking for, please feel free to get in touch and we will do our best to find what you need. Alternatively search our website.

Not purchased through Caltag Medsystems before? Learn more about our company on ourAbout Us page or view our Testimonials page.

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